Commercial Probiotic Drink and Sumbawa Horse Milk as Lactic Acid Bacteria Source for Virgin Coconut Oil Extraction
Abstract
Virgin coconut oil (VCO) can be produced without heat, without any alteration, requires minimal investments and energy during production, and has been reported to have many health benefits. We produced VCO with the addition of lactic acid bacteria (LAB) sources with combined method of chilling-thawing and fermentation. The LAB sources used were Sumbawa horse milk (SKL) and commercial probiotic milk (SPK). This study used a completely randomized design (CRD) using both LAB sources which were added to coconut milk, respectively 10 ml, 12.5 ml and 15 ml and compared with control or no treatment. LAB were also isolated from blondo and VCO using LAB specific medium, MRSA. The extracted VCO has the characteristics of clear color with a distinctive coconut smell and long shelf-life. Three LAB isolates were obtained, VCO1-3. VCO1 was found in all samples, with colony morphology of circular and entire margin, convex surface, small in size, and white in color. SKL10, SKL15 and SPK10 showed significant difference to the control, with VCO yield of 31.49-33.05 %, significantly higher than that of without LAB treatment. Both LAB sources of single (SPK) and mixed inoculums (SKL) showed higher yields, indicated their potential as starter for VCO extraction.
Keywords - Virgin Coconut Oil, VCO, Chilling-Thawing Extraction, Fermentation Extraction, Cold Pressed Oil, Lactic Acid Bacteria.
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A. N. Aulia, N. Chasanah, A. S. Prasetyo, and A. N. Nalawati, “Competitiveness and Export Similarity of Indonesia’s Coconut Oil,” Jurnal Agribest, vol. 4, no. 2, pp. 123–132, Oct. 2020, doi: 10.32528/AGRIBEST.V4I2.3546.
J. C. Alouw and S. Wulandari, “Present status and outlook of coconut development in Indonesia,” IOP Conf Ser Earth Environ Sci, vol. 418, no. 1, p. 012035, Jan. 2020, doi: 10.1088/1755-1315/418/1/012035.
“Virgin Coconut Oil Market Size & Growth | Forecast [2021-2028].” https://www.fortunebusinessinsights.com/virgin-coconut-oil-market-106554 (accessed Dec. 30, 2022).
“Badan Pusat Statistik.” https://www.bps.go.id/indicator/54/132/1/produksi-tanaman-perkebunan.html (accessed Jan. 18, 2023).
I. Permatasari, “Etnobotani Tumbuhan Bahan Dasar Minyak Sumbawa di Kabupaten Sumbawa Besar Provinsi Nusa Tenggara Barat (NTB),” UIN Malang, Malang, 2013.
A. Rohman et al., “Virgin Coconut Oil: Extraction, Physicochemical Properties, Biological Activities and Its Authentication Analysis,” Food Reviews International, vol. 37, no. 1. Bellwether Publishing, Ltd., pp. 46–66, 2021. doi: 10.1080/87559129.2019.1687515.
N. A. A. Ghani, A. A. Channip, P. Chok Hwee Hwa, F. Ja’afar, H. M. Yasin, and A. Usman, “Physicochemical properties, antioxidant capacities, and metal contents of virgin coconut oil produced by wet and dry processes,” Food Sci Nutr, vol. 6, no. 5, pp. 1298–1306, Jul. 2018, doi: 10.1002/FSN3.671.
S. V. Ramesh et al., “Engineering intervention for production of virgin coconut oil by hot process and multivariate analysis of quality attributes of virgin coconut oil extracted by various methods,” J Food Process Eng, vol. 43, no. 6, p. e13395, Jun. 2020, doi: 10.1111/JFPE.13395.
Y. Srivastava, A. D. Semwal, and A. Majumdar, “Quantitative and qualitative analysis of bioactive components present in virgin coconut oil,” Cogent Food Agric, vol. 2, no. 1, 2016, doi: 10.1080/23311932.2016.1164929.
A. R. Fathur, Y. Hendrawan, S. Rosalia Dewi, and S. Malin Sutan, “Optimasi Nilai Rendemen dalam Pembuatan Virgin Coconut Oil (VCO) Menggunakan Pemanasan Suhu Rendah dan Kecepatan Sentrifugasi dengan Response Surface Methodology (RSM),” Jurnal Keteknikan Pertanian Tropis dan Biosistem, vol. 6, no. 3, pp. 218–228, Sep. 2018, Accessed: Jan. 02, 2023. [Online]. Available: https://jkptb.ub.ac.id/index.php/jkptb/article/view/478
K. G. Nevin and T. Rajamohan, “Virgin coconut oil supplemented diet increases the antioxidant status in rats,” Food Chem, vol. 99, no. 2, pp. 260–266, 2006, doi: 10.1016/j.foodchem.2005.06.056.
A. Narayanankutty, S. P. Illam, and A. C. Raghavamenon, “Health impacts of different edible oils prepared from coconut (Cocos nucifera): A comprehensive review,” Trends in Food Science and Technology, vol. 80. Elsevier Ltd, pp. 1–7, Oct. 01, 2018. doi: 10.1016/j.tifs.2018.07.025.
U. Patil and S. Benjakul, “Coconut Milk and Coconut Oil: Their Manufacture Associated with Protein Functionality,” Journal of Food Science, vol. 83, no. 8. Blackwell Publishing Inc., pp. 2019–2027, Aug. 01, 2018. doi: 10.1111/1750-3841.14223.
S. N. Raghavendra and K. S. M. S. Raghavarao, “Effect of different treatments for the destabilization of coconut milk emulsion,” J Food Eng, vol. 97, no. 3, pp. 341–347, Apr. 2010, doi: 10.1016/J.JFOODENG.2009.10.027.
T. Aziz, Y. Olga, and A. P. Sari, “Pembuatan virgin coconut oil (vco) Dengan metode penggaraman,” Jurnal Teknik Kimia, vol. 23, no. 2, pp. 129–136, Apr. 2017, Accessed: Oct. 17, 2022. [Online]. Available: http://ejournal.ft.unsri.ac.id/index.php/JTK/article/view/751
A. M. Marina, Y. B. Che Man, S. A. H. Nazimah, and I. Amin, “Chemical properties of virgin coconut oil,” JAOCS, Journal of the American Oil Chemists’ Society, vol. 86, no. 4, pp. 301–307, Apr. 2009, doi: 10.1007/S11746-009-1351-1/TABLES/4.
M. Shilling et al., “Antimicrobial effects of virgin coconut oil and its medium-chain fatty acids on clostridium difficile,” J Med Food, vol. 16, no. 12, pp. 1079–1085, Dec. 2013, doi: 10.1089/jmf.2012.0303.
N. Noriko, A. Masduki, R. Azhari, G. Nufadianti, P. Studi Biolgi, and F. Sains danTeknologi, “Uji In Vitro Daya Anti Bakterial Virgin Coconut Oil (VCO) pada Salmonella typhi,” JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI, vol. 2, no. 3, pp. 188–192, Nov. 2015, Accessed: Mar. 21, 2023. [Online]. Available: https://jurnal.uai.ac.id/index.php/SST/article/view/140
T. H. Chen, M. Ahmad1, N. Akhmazillah, and M. Fauzi, “Comparison Study on Antimicrobial Activity and Antimicrobial Agent Release of Virgin Coconut Oil (VCO) and Lauric Acid Modified Pullulan/Starch Based Films,” Progress in Engineering Application and Technology, vol. 1, no. 1, pp. 378–389, 2020, doi: 10.30880/peat.2020.01.01.040.
A. A. Anzaku, “Antimicrobial Activity of Coconut Oil and its Derivative (Lauric Acid) on Some Selected Clinical Isolates,” International Journal of Medical Science and Clinical Inventions, vol. 4, no. 8, Aug. 2017, doi: 10.18535/ijmsci/v4i8.12.
W. M. de Azevedo et al., “Physicochemical characterization, fatty acid profile, antioxidant activity and antibacterial potential of cacay oil, coconut oil and cacay butter,” PLoS One, vol. 15, no. 4, Apr. 2020, doi: 10.1371/journal.pone.0232224.
N. Satoshi et al., “Comparison of the antimicrobial spectrum and mechanisms of organic virgin coconut oil and lauric acid against bacteria,” Journal of Wellness and Health Care, vol. 41, no. 1, pp. 87–95, 2017.
I. Angeles-Agdeppa, J. S. Nacis, M. V. Capanzana, F. M. Dayrit, and K. V. Tanda, “Virgin coconut oil is effective in lowering C-reactive protein levels among suspect and probable cases of COVID-19,” J Funct Foods, vol. 83, p. 104557, Aug. 2021, doi: 10.1016/J.JFF.2021.104557.
M. Siddalingaswamy, A. Rayaorth, and F. Khanum, “Anti-diabetic effects of cold and hot extracted virgin coconut oil,” J Diabetes Mellitus, vol. 01, no. 04, pp. 118–123, 2011, doi: 10.4236/jdm.2011.14016.
M. A. Darmawan, K. Siregar, and M. Gozan, “Coconut Oil,” Biorefinery of Oil Producing Plants for Value‐Added Products, pp. 99–122, Jan. 2022, doi: 10.1002/9783527830756.CH6.
S. Lockyer and S. Stanner, “Coconut oil - a nutty idea?,” Nutr Bull, vol. 41, no. 1, pp. 42–54, Mar. 2016, doi: 10.1111/nbu.12188.
A. Osman, “Coconut (Cocos nucifera) Oil,” in Fruit Oils: Chemistry and Functionality, Springer International Publishing, 2019, pp. 209–221. doi: 10.1007/978-3-030-12473-1_9.
M. L. Assunção, H. S. Ferreira, A. F. Dos Santos, C. R. Cabral, and T. M. M. T. Florêncio, “Effects of dietary coconut oil on the biochemical and anthropometric profiles of women presenting abdominal obesity,” Lipids, vol. 44, no. 7, pp. 593–601, Jul. 2009, doi: 10.1007/s11745-009-3306-6.
G. C. Gervajio, T. S. Withana‐Gamage, and M. Sivakumar, “Fatty Acids and Derivatives from Coconut Oil,” in Bailey’s Industrial Oil and Fat Products, Wiley, 2020, pp. 1–45. doi: 10.1002/047167849x.bio039.pub2.
S. Suryani et al., “A Comparative Study of Virgin Coconut Oil, Coconut Oil and Palm Oil in Terms of Their Active Ingredients,” Processes 2020, Vol. 8, Page 402, vol. 8, no. 4, p. 402, Mar. 2020, doi: 10.3390/PR8040402.
F. M. Dayrit, “The Properties of Lauric Acid and Their Significance in Coconut Oil,” J Am Oil Chem Soc, vol. 92, no. 1, pp. 1–15, Jan. 2015, doi: 10.1007/s11746-014-2562-7.
C. B. J. Villarino, A. R. P. Basinang, M. M. M. Velasquez, J. M. DG. Pagulayan, P. K. A. Ong, and Ma. C. C. Lizada, “Descriptive Aroma Changes in Selected Philippine Virgin Coconut Oil (VCO) during Storage at Elevated Temperatures,” Proceedings 2021, Vol. 70, Page 111, vol. 70, no. 1, p. 111, Nov. 2020, doi: 10.3390/FOODS_2020-07736.
A. Rahmadi, A. Ipnatul, M. D. Sukarno, and T. Purnaningsih, “Karakteristik Fisikokimia dan Antibakteri Virgin Coconut Oil Hasil Fermentasi Bakteri Asam Laktat,” Jurnal Teknologi dan Industri Pangan, vol. 24, no. 2, pp. 178–178, Dec. 2013, doi: 10.6066/JTIP.2013.24.2.178.
K. A. Fidien et al., “Diversity analysis, identification, and bioprospecting of Lactic Acid Bacteria (LAB) isolated from Sumbawa horse milk,” Biodiversitas, vol. 22, no. 6, pp. 3333–3340, May 2021, doi: 10.13057/BIODIV/D220639.
Kusdianawati, A. Z. Mustopa, Fatimah, and B. R. Budiarto, “Genetic diversity of lactic acid bacteria isolated from Sumbawa horse milk, Indonesia,” Biodiversitas, vol. 21, no. 7, pp. 3225–3233, Jun. 2020, doi: 10.13057/BIODIV/D210744.
K. A. Ravindra and S. Bosco, “Extraction Processes of Virgin Coconut Oil,” MOJ Food Processing & Technology, vol. 4, no. 2, pp. 54–56, 2017, doi: 10.15406/mojfpt.2017.04.00087.
Y. Miftasari, “Produksi dan Karakteristik Fisikokimia serta Bioaktivitas Minyak Kelapa Hasil Fermentasi Lactobacillus casei,” 2017, Accessed: Sep. 19, 2022. [Online]. Available: http://repository.ipb.ac.id/handle/123456789/89722.
T. Aziz, Y. Olga, and A. Puspita Sari, “PEMBUATAN VIRGIN COCONUT OIL (VCO) DENGAN METODE PENGGARAMAN,” 2017.
D. Andrianto, L. al Rasyid, and Y. Miftasari, “Physicochemical Characteristics and Bioactivity of Lactobacillus casei Fermented Coconut Oil,” IOP Conf Ser Earth Environ Sci, vol. 197, no. 1, p. 012003, Oct. 2018, doi: 10.1088/1755-1315/197/1/012003.
A. F. Mulyadi, M. Schreiner, and I. A. Dewi, “Phenolic and volatile compounds, antioxidant activity, and sensory properties of virgin coconut oil: Occurrence and their relationship with quality,” in AIP Conference Proceedings, American Institute of Physics Inc., Oct. 2018. doi: 10.1063/1.5062818.
N. T. Oseni, W. Fernando, R. Coorey, I. Gold, and V. Jayasena, “Effect of extraction techniques on the quality of coconut oil,” African Journal of Food Science, vol. 11, no. 3, pp. 58–66, Mar. 2017, doi: 10.5897/ajfs2016.1493.
D. Arisanti and I. O. Angelia, “Peningkatan Kualitas Minyak Kelapa Murni (VCO) Terfermentasi Kultur Kering Bakteri Asam Laktat (BAL) terhadap Rendemen dan Kadar Air,” Jurnal Pertanian, vol. 11, no. 1, pp. 21–24, Apr. 2020, doi: 10.30997/JP.V11I1.2178.
A. F. Mulyadi, M. Schreiner, and I. A. Dewi, “An overview of factors that affected in quality of virgin coconut oil,” AIP Conf Proc, vol. 2120, no. 1, p. 050007, Jul. 2019, doi: 10.1063/1.5115683.
N. Asiah, R. Astuti, L. Cempaka, and R. Setiani, “Physical and Chemical Characteristic of Virgin Coconut Oil under Mix Culture Fermentation Technique,” J Phys Conf Ser, vol. 1364, no. 012009, pp. 1–8, 2019, doi: 10.1088/1742-6596/1364/1/012009.
T. S. T. Mansor, C. Man, A. Afiq, and K. Nurul, “Physicochemical properties of virgin coconut oil extracted from different processing methods,” Int Food Res J, vol. 19, no. 3, pp. 837–845, 2012.
Z. A. Maini and C. M. Lopez, “Transitions in bacterial communities across two fermentation-based virgin coconut oil (VCO) production processes,” Heliyon, vol. 8, no. 8, p. e10154, Aug. 2022, doi: 10.1016/J.HELIYON.2022.E10154.
DOI: http://dx.doi.org/10.36722/sst.v8i3.1904
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