Analisis Bioinformatika Berbasis WEB untuk Eksplorasi Enzim Kitosanase Berdasarkan Kemiripan Sekuens
Abstract
Abstract
Enzymes exploration which is traditionally conducted by microbial cultivation, is no longer efficient, for spending the time and cost. Web based bioinformatics presents to do a series of sequence analysis, for query both DNA and protein, which can be used as preliminary test in order to direct the research effectively. We have conducted an analysis of 16S ribosomal RNA sequences from 6 bacteria in association with shrimp. The goal is finding out the recording in Gene Banks, which if they have not recorded means they are Indonesian strain. Using the 16S Microbial and Reference Genomic Sequence databases, as well as BLAST nucleotide and CLUSTALW2, we obtained 5 names of bacteria, i.e., Micromonospora sp. L5, Aeromonas veronii B565, Staphylococcus epidermidis ATCC 12228, Burkholderia sp. JV3, and Acinetobacter baumannii AB307-0294. These microbes do not have the chitosanase gene but have chitinase gene. The absence of chitosanase gene is unique its sequence that also gives opportunity for publication.
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PREVIEW DOCUMENTDOI: http://dx.doi.org/10.36722/sst.v1i4.84
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LP2M (Lembaga Penelitian dan Pengembangan Masyarakat)
Universitas AL-AZHAR INDONESIA, Lt.2 Ruang 207
Kompleks Masjid Agung Al Azhar
Jl. Sisingamangaraja, Kebayoran Baru
Jakarta Selatan 12110
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