Gambaran Patologi Embrio Ayam yang Terinfeksi Avian orthoreovirus Isolat Lokal

Raditya Pradana Putra, Sri Murtini, Okti Nadia Poetri

Abstract


Avian orthoreovirus (ARV) is widespread and found in almost every commercial poultry farm and other poultry species. Virus isolation from local isolates is necessary to obtain vaccine seeds that are homologous to viruses in the field.  The R&D Laboratory of PT Vaksindo Satwa Nusantara successfully isolated ARV from field cases. Inoculation of SPF Embryonated Chicken Eggs (ECE) is the protocol used to evaluate virus titer, especially to calculate the antigen titer of vaccine prototype candidates. The gross pathology of chicken embryos infected with ARV to evaluate virus growth has not been widely reported. This study aims to observe the gross pathology of chicken embryos infected with ARV to measure the virus titer using infectivity test such as the embryo infectious dose 50 (EID50). The virus suspension was diluted from 10-1 to 10-6 then dilutions of 10-4 to 10-6 were inoculated each into five ECE. Incubated ten days and observed for embryonic death. The observations found the embryonic death on day seven. Pathological changes in the embryos are stunted, thin feather, oedema, and less allantois fluid compared to control of uninfected embryo. The results of the embryos necropsy showed cardiac necrosis, greenish liver, and gelatinous exudate in the abdominal cavity.

Keywords - Avian Orthoreovirus, Embryonated Chicken Eggs, Embryo Pathology, EID50.


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References


L. van der Heide, ‘The History of Avian Reovirus’, Avian Dis, vol. 44, no. 3, pp. 638–641, 2000, doi: 10.2307/1593104.

J. Pitcovski and S. M. Goyal, ‘Avian reovirus infections’, Diseases of Poultry, pp. 382–400, 2020.

H. Lu, Y. Tang, P. A. Dunn, E. A. Wallner-Pendleton, L. Lin, and E. A. Knoll, ‘Isolation and molecular characterization of newly emerging avian reovirus variants and novel strains in Pennsylvania, USA, 2011–2014’, Sci Rep, vol. 5, no. 1, pp. 1–11, 2015.

F. S. B. Kibenge and G. E. Wilcox, ‘Tenosynovitis in chickens’, Veterinary Bulletin, vol. 53, no. 5, pp. 431–444, 1983.

A. W. Kalupahana, ‘Characterization of orthoreoviruses isolated from American crow (Corvus brachyrhynchos) winter mortality events in eastern Canada’, 2017.

R. C. Jones, ‘Reovirus Infections’, in Diseases of Poultry, 2013, pp. 351–373. doi: https://doi.org/10.1002/9781119421481.ch11.

D. N. Dharma, P. Darmadi, K. A. P. Santhia, and I. G. Sudhana, ‘Studi penyakit helikopter pada ayam pedaging’, in Pros. Seminar Peternakan dan Forum Peternak Unggas dan Aneka Ternak. Bogor, 1985, pp. 19–20.

R. Ernawati, ‘Penyakit Arthritis Pada Ayam Di Beberapa Peternakan Di Daerah Malang Dan Sekitarnya Usaha Isolasi Dan Identifikasi Agen Penyebabnya’, 1993.

S. WAHYUWARDANI, H. Huminto, and L. Parede, ‘Perubahan patologi secara makroskopi dan mikroskopi pada ayam pedaging yang diinfeksi reovirus isolat lokal’, JITV, vol. 10, no. 1, 2005, Accessed: Feb. 17, 2022. [Online]. Available: https://balitnak.litbang.pertanian.go.id/phocadownload/JITV/63-70a.pdf.

R. Hartawan and N. L. P. I. Dharmayanti, ‘Detection of Fife Virus Infections in The Layer Farm with Runting-Stunting Syndrome in Sukabumi and Tangerang Using Polymerase Chain Reaction Technique’, Jurnal Kedokteran Hewan June, vol. 11, no. 2, pp. 65–69, 2017.

D. Qosimah, S. Murwani, and I. Amalia, Penyakit Viral pada Unggas. Universitas Brawijaya Press, 2017.

R. C. Jones, “Avian reovirus infections,” Revue Scientifique et Technique-Office International des Epizooties, vol. 19, no. 2, hlm. 614–619, 2000.

S. M. Mansour, R. M. ElBakrey, A. Orabi, H. Ali, and A. A. Eid, ‘Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission’, Journal of Virological Sciences, vol. 4, pp. 24–32, 2018.

M. A. Ramakrishnan, ‘Determination of 50% endpoint titer using a simple formula’, World J Virol, vol. 5, no. 2, p. 85, 2016.




DOI: http://dx.doi.org/10.36722/sst.v9i1.2143

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