Deteksi Keberadaan Antigen dan Antibodi terhadap Equine Influenza pada Kuda Impor dari Belanda

Amrie Muhammad Alzab, Sri Murtini, Eko Sugeng Pribadi

Abstract


Equine Influenza (EI) is a highly contagious respiratory infection disease in equidae caused by two subtypes of Influenza A Virus (H7N7 and H3N8). Indonesia is recently importing high amounts of horses from The Netherlands intended for equestrian sports, pets, and breeders. This study was aimed to discover the presence of Equine Influenza Virus (EIV) and antibody titres against EIV in imported horses from the Netherlands using Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Enzyme-linked Immunosorbent Assay (ELISA) techniques. The results obtained were then compared to imported horses’ data. A total of 199 nasopharyngeal swabs and 55 blood samples were collected. The results showed no material genetic of EIV was found in horses imported from the Netherlands, not all recently imported horses had antibodies against EIV, it’s indicated that the imported horses need to be measured the antibody post vaccination before arrived in Indonesia.

Keywords - Horse, Equine Influenza, The Netherlands, PCR, ELISA


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References


R.K. Singh, K. Dhama, K. Karthik, R. Khandia, A. Munjal, S.K. Khurana, S. Chakraborty, Y.S. Malik, N. Virmani, R. Singh, B.N. Tripathi, M. Munir, J.H. van der Kolk, A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies Front. Microbiol. 9: p.1941, 2018. DOI:10.3389/fmicb.2018.01941.

A. Khan, M.H. Mushtaq, J. Muhammad, B. Ahmed, E.A. Khan, A. Khan, S.A. Zakki, E. Altaf, I. ul Haq, A. Saleem, M.A. Warraich, N. Ahmed, A.A. Rabaan, Global Epidemiology of Equine Influenza Viruses: A Possible Emerging Zoonotic Threat in Future, An Extensive Systemic Review with Evidence, Brazilian Journal of Biology, 83: e246591, 2021.

A. Cullinane, Equine Influenza and Air Transport, Equine Vet Educ, 26(9): p.456-457, 2014. DOI:10.1111/eve.12215.

World Organisation for Animal Health, WOAH Terrestrial Manual Chapter 3.5.7: Equine Influenza (Infection with Equine Influenza Virus), https://www.woah.org/en/what-we-do/standards/codes-and-manuals/terrestrial-code-online-access/, 2019. (Diakses pada 6 November 2021)

World Organisation for Animal Health, WOAH Terrestrial Manual Chapter 3.6.7: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/A_summry.htm, 2019. (Diakses pada 6 November 2021)

World Organisation for Animal Health, World Animal Health Information System, https://wahis.woah.org/#/dashboards/country-or-disease-dashboard, 2022. (Diakses pada 16 Desember 2022)

Badan Pusat Statistik, Populasi Kuda Menurut Provinsi, https://www.bps.go.id/indicator/24/475/1/populasi-kuda-menurut-provinsi.html, 2021. (Diakses pada 6 November 2021)

Kementerian Pertanian Republik Indonesia, Keputusan Menteri Pertanian Nomor 3238/Kpts/PD.630/9/2009 tentang Penggolongan Jenis-Jenis Hama Penyakit Hewan Karantina Penggolongan dan Klasifikasi Media Pembawa, Jakarta, 2009.

Kementerian Pertanian Republik Indonesia, Pusat Data dan Sistem Informasi Pertanian, http://database.pertanian.go.id/eksim/index1.asp, 2021. [Diakses pada 9 April 2022]

Badan Karantina Pertanian, Data Komoditas Hewan Impor BBKP Soekarno-Hatta, Jakarta, IQ-FAST, 2021.

C. Wang, Q. Wang, J. Hu, H. Sun, J. Pu, J. Liu, Y. Sun, A Multiplex RT-PCR Assay for Detection and Differentiation of Avian-Origin Canine H3N2, Equine-Origin H3N8, Human-Origin H3N2, and H1N1/2009 Canine Influenza Virus, Journal PloS One, 12 (1): p.1-12, 2017. DOI:10.1371/journal.pone.0170374.

S. Aeschbacher, E. Santschi, V. Gerber, H.P. Stalder, R.G. Zanoni, Development of a Real-time RT-PCR for Detection of Equine Influenza Virus, Schweiz Arch Tierheilkd, 157: p.191-201, 2015. DOI:10.17236/sat00015.

Pemerintah Republik Indonesia, Peraturan Pemerintah No. 82 Tahun 2000 tentang Karantina Hewan, Jakarta, 2000.

Pemerintah Republik Indonesia, Undang-Undang No. 21 Tahun 2019 tentang Karantina Hewan, Ikan, dan Tumbuhan, Jakarta, 2019.

P. Galvin, S. Gildea, S. Arkins, C. Walsh, A. Cullinane, The Evaluation of Nucleoprotein ELISA for the Detection of Equine Influenza Antibodies and the Differentiation of Infected from Vaccinated Horses (DIVA), Influenza Other Respir. Viruses, 7: p.73-80, 2013. DOI:10.1111/irv.12195.

R. Kittelberger, A.M. McFadden, M.J. Hannah, Comparative Evaluation of Four Competitive/Blocking ELISAs for the Detection of Influenza A Antibodies in Horses, Vet. Microbiol. 148: p.377-83, 2011. DOI:10.1016/j.vetmic.2010.08.014.

J.A. Mumford, D.M. Jessett, E.A. Rollinson, D. Hannant, M.E. Draper, Duration of Protective Efficacy of Equine Influenza Immunostimulating Complex/Tetanus Vaccines, Vet Record. 134(7): p.158-162, 1994. DOI:10.1136/vr.134.7.158. PMID: 8160328.

F.S. Oladunni, S.O. Oseni, L. Martinez-Sobrido, T.M. Chambers, Equine Influenza Virus and Vaccines, Viruses, 13: p.1657, 2021. DOI:10.3390/v13081657.




DOI: http://dx.doi.org/10.36722/sst.v9i1.2117

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