Nested Polymerase Chain Reaction (Nested-PCR) As A Diagnostic Technique for Intestinal Helminth Infection

Reqgi First Trasia


Abstract - Worm infections are still a health problem in the world, involving Indonesia. This disease generally rarely causes death, it has a detrimental impact on society such as malnutrition, anemia, productivity declining, and inhibits physical growth and intelligence in children. Efforts to support the intestinal helminth infection elimination program must be supported by proper diagnosis so that it can be continued with appropriate treatment and as a guideline for determining further policy. Microscopic examination is currently still the most commonly used method but has a limitation, especially less sensitive if in conditions of low infection. Molecular diagnoses suc [1]h as PCR can provide an alternative in providing a more sensitive and specific diagnosis of intestinal helminth infection.

Keywords - Diagnosis, Intestinal helminth infections, Polymerase Chain Reaction

Full Text:



World Health Organization, Februari 2018. [Online]. Available:

Kementrian Kesehatan Republik Indonesia, Peraturan Menteri Kesehatan Republik Indonesia Nomor 15 Tahun 2017 tentang Penanggulangan Kecacingan, S. F. d. Kecacingan, Penyunt., Jakarta: Kementrian Kesehatan Republik Indonesia, 2017.

Anorital, R. M. Dewi dan K. Palupi, “Studi kajian Upaya Pemberian Obat Pencegah Masal Filarisasi Terhadap Pengendalian Penyakit Infeksi Kecacingan,” Jurnal Biotek Medisiana Indonesia, vol. 5, no. 2, pp. 95 -103, 2016.

R. Anderson, S. Farrel, H. Turner, J. Walson dan C. A. D. &. J. Truscott, “Assessing the interruption of the transmission of human helminths with mass drug administration alone: optimizing the design of cluster randomized trials,” Parasites & Vectors, vol. 10, no. 93, 2017.

R. M. Anderson, H. C. Turner, J. E. Truscott, T. D. Hollingsworth dan S. J. Brooker, “Should the Goal for the Treatment of Soil Transmitted Helminth (STH) Infections Be Changed from Morbidity Control in Children to Community-Wide Transmission Elimination?,” Plos Neglected Tropical Diseases, vol. 9, no. 8, August 2015.

M. D. Lim, S. J. Brooker, V. Y. Jr Belizario, et all, “Diagnostic tools for soil-transmitted helminths control and elimination programs: A pathway for diagnostic product development,” Plos Neglected Tropical Diseases, vol. 12, no. 3, Maret 2018.

J. C. Dunn, H.C. Turner, A. Tun, R. M. Anderson, “Epidemiological surveys of, and research on, soil-transmitted helminths in Southeast Asia: a systematic review,” Parasit Vectors, vol. 9, no. 31, Januari 2016.

M. Werkman, J. E. Wright, J. E. Truscott et all, “Testing for soil-transmitted helminth transmission elimination: Analysing the impact of the sensitivity of different diagnostic tools,” Plos Neglected Tropical Diseases, vol. 12, no. 1, Januari 2018.

L. v. Lieshout dan M. Yazdanbakhsh, “Landscape of neglected tropical diseases: getting it right,” Lancet Infect Dis, vol. 13, no. 6, 2013.

J. J. Verweij, E. A. T. Brienen, J. Ziem, L. Yelifari, A. M. Polderman dan L. V. Lieshout, “Simultaneous Detection and Quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in Fecal Samples Using Multiplex Real-Time PCR,” The American Journal of Tropical Medicine and Hygiene, vol. 77, no. 4, pp. 685-690, 2007.

I. Sutanto, I. S. Ismid, P. K. Sjarifuddin dan S. Sungkar, Buku ajar parasitologi kedokteran, Jakarta: Balai Penerbit FKUI, 2008.

R. Subahar, P. Patiah, A. U. widiastuti dan H. Wibowo, “Pravelensi dan Intensitas Infeksi Ascaris Lumbricoides dan Trichuris trichiura pada Anggota Keluarga di Jaarta dan Cipanas, Jawa Barat,” Jurnal Profesi Nedika, vol. 11, no. 1, 2017.

Central for Diease Control and Prevention, [Online]. Available: [Diakses 20 Juni 2020].

World Health Organization, “Soil-transmitteshelminthiases: eliminanting soil transmitted helminthiases as a public helath problem in childern: progress report 2001-2010 andstrategic plan 2011-2020,” WHO, Prancis, 2012.

T. Ni'mah, “Diagnosis STH Menggunakan PCR. Makalah Seminar Umum,” 2-26, 2019



  • There are currently no refbacks.

LP2M (Lembaga Penelitian dan Pengembangan Masyarakat)

Universitas AL-AZHAR INDONESIA, Lt.2 Ruang 207

Kompleks Masjid Agung Al Azhar

Jl. Sisingamangaraja, Kebayoran Baru

Jakarta Selatan 12110