Pengaruh Penambahan Berbagai Konsentrasi L-Ascorbic Acid Terhadap Kemampuan Migrasi Adipose-Derived Stem Cells Asal Manusia

Karina Karina, Imam Rosadi, Wismo Reja Subroto, Alfida Zakiyah, Irsyah Afini, Iis Rosliana, Tias Widyastuti, Siti Sobariah

Abstract


Abstrak - Mesenchymal Stem Cells (MSCs) merupakan sel yang memiliki kemampuan memperbarui diri dan berdiferensiasi menjadi berbagai jenis sel. MSCs berperan penting dalam perbaikan dan regenerasi jaringan. Adipose-Derived Stem Cells (ADSCs) merupakan MSCs yang didapatkan dari jaringan lemak. ADSCs telah banyak digunakan dalam studi MSCs karena ketersediaan sumber sel yang banyak dalam jaringan tubuh dan proses isolasinya lebih mudah serta memiliki risiko yang lebih rendah. Pada studi ini, ADSCs digunakan untuk mengetahui kemampuan migrasi dalam medium yang mengandung fetal bovine serum 10% (FBS) dengan penambahan L-Ascorbic Acid (LAA) konsentrasi 0%, 1%, 3% dan 6%. Kuantifikasi kemampuan migrasi dilakukan dengan menggunakan teknik goresan luka. ADSCs yang dikulturkan menunjukkan morfologi seperti fibroblas. Luas penutupan luka menunjukkan hasil tertinggi dengan perbedaan yang tidak signifikan pada penambahan konsentrasi LAA 3% dan 6% dibandingkan penambahan LAA 0%, 1% dan kontrol. Hasil ini juga ditunjukkan pada pasase 7, 8, dan 9. ADSCs menunjukkan kemampuan migrasi yang optimal pada penambahan LAA 3% dan 6% dibandingkan medium dengan penambahan LAA 0%, 1% dan kontrol.

Abstract - Mesenchymal stem cells (MSCs) are multilineage cells with the ability to self-renew and differentiate into a variety of cell types. MSCs play important role in tissue repair and regeneration. Adipose-Derived Stem Cells (ADSCs) are MSCs derived from fat tissue. ADSCs has been widely used in MSCs studies because it require more cell sources in body tissue and the process of isolation is easier and has lower risk. In this study, ADSCs were used to determine migration ability in a medium containing 10% bovine fetal serum (FBS) using 0%, 1%, 3% and 6% of L-Ascorbic Acid (LAA) concentrations. Quantification of migration ability was done by using scratch technique. ADSCs were cultured display fibroblasts-like morphology. The closure of wound area is highest with not significantly difference in LAA concentrations of 3% and 6% compared to LAA 0%, 1% and control. This results were also showed on 7, 8, and 9 cell passage. ADSCs showed optimal migration ability at LAA 3% and 6% compared to media with LAA 0%, 1% and control.

Keywords - ADSCs, Cells Migration, LAA.


Full Text:

PDF

References


I. Rosadi et al., “In vitro study of cartilage tissue engineering using human adipose-derived stem cells induced by platelet-rich plasma and cultured on silk fibroin scaffold,” Stem Cell Research & Therapy, vol.10, no.1, pp.1-15, 2019.

Karina et al., “Diabetes mellitus type 2 reduces the viability, proliferation, and angiogenic marker of adipose-derived stem cells cultured in low-glucose anti-oxidant-serum supplemented medium,” Biomedical Research and Therapy, vol.6, no.3 pp.3073-3082, 2019.

X. Fu, G. Liu, A. Halim, Y. Ju, Q. Luo, dan G. Song, “Mesenchymal Stem Cell Migration and Tissue Repair,” Cells, vol.8, no.8, pp.784, 2019.

G. F. Nicoletti, F. De Francesco, F. D’Andrea, dan G.A. Ferraro, “Methods and procedures in adipose stem cells: state of the art and perspective for translation medicine,” J. Cell. Physiol, vol.230, no.3, pp.489-495, 2015.

L. Mazini, L. Rochette, S. Amal, B. Admou, dan G. Malka, “Adipose Derived Stem Cells (ADSCs) Immunomodulation Impact on Skin Tissue Repair,” Journal of Embryology & Stem Cell Research, ISSN: 2640-2637, 2020.

S. J. Zhang, X. Y. Song, M. He, dan S.B. Yu, “Effect of TGF-β1/SDF 1/CXCR4 signal on BM-MSCs homing in Rat Heart of Ischemia/Perfusion Injury,” Eur. Rev. Med. Pharmacol. Sci, vol.20, no.5, pp.899–905, 2016.

A. C. Brignier dan A.M. Gewirtz. “Embryonic and adult stem cell therapy,” J. Allergy Clin Immunol, vol.125, pp.S336-344, 2010.

J. N. Barker dan J. E. Wagner, “Umbilical cord blood transplantation: current practice and future innovation,” Crit Rev Oncol Hematol. vol.48, no.1, pp.35-43, 2003.

P. A. Zuk et al., “Human adipose tissue is a source of multipotent stem cells,” Mol Biol Cell, vol.13, no.12, pp.4279-4295, 2002.

B. Lindroos, R. Suuronen, dan S. Miettinen, “The Potential of Adipose Stem Cells in Regenerative Medicine,” Stem Cell Rev and Rep, vol.7, no.2, pp. 269-291, 2011.

Y. Zhu, T. Liu, K. Song, X. Fan, X. Ma, dan Z. Cui, “Adipose‐derived stem cell: a better stem cell than BMSC,” Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease, vol.26, no.6, pp.664-675, 2008.

Hu J, Cheng D, Gao X, Bao J, Ma X, dan Wang H, “Vitamin C enhances the in vitro development of porcine pre-implantation embryos by reducing oxidative stress,” Reprod Domest Anim, vol.47, no.6, pp.873 879, 2012.

K. Fujisawa, K. Hara, T. Takami, S. Okada, T. Matsumoto, N. Yamamoto, dan I. Sakaida. “Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells,” Stem Cell Research & Therapy, vol. 9, no. 1, 2018.

I. Rosadi dan K. Moegni, “Perbandingan Kemampuan Migrasi Adipose-Derived Stem Cells Asal Manusia pada Berbagai Medium Pertumbuhan,” Bioeksperimen, vol.6, no.1, pp.45-51, 2020.

P. Y. Yue, E. P. Leung, N. K. Mak, dan R. N. Wong, “A simplified method for quantifying cell migration/wound healing in 96-well plates,” J. Biomol. Screening, vol. 15, no.4, pp.427-433, 2010.

K. Moegni et al., “Stromal vascular fraction (SVF) therapy for treatment of various diseases: delivering safety of the first patented svf technique in Indonesia,” Cytotherapy, vol.21, no.5, 2019.

M. Dominici et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy. vol. 8, no.4, pp.315-317, 2006.

N. Kramer et al., “In vitro cell migration and invasion assays,”Mutation Research/Reviews in Mutation Research, vol.752, no.1, pp.10-24, 2013.

C. C. Liang, A. Y. Park, dan J. L. Guan. “In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro,” Nature protocols, vol.2, no.2, pp.329, 2007.

H. Büth et al., “Cathepsin b is essential for regeneration of scratch wounded normal human epidermal keratinocytes,” European Journal of Cell Biology, vol.86, no.11-12, pp.747–761, 2007.

A. V. P. Bobadilla et al., “Local migration quantification method for scratch assays,” J. R. Soc. Interface, vol.16, no.151, 2018.

W. Wagner et al., “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PloS One, vol.3, no.5, 2008.

E. Fathi dan R. Farahzadi, “Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview,” Brazilian Archives of Biology and Technology, vol.59, 2016.

I. Rosadi et al., “The Effect of Human Platelet-Rich Plasma and L Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells,” Molecular and Cellular Biomedical Sciences, vol.3, no.1, 2019.

R. D. Horwitz dan Webb, “Cell Migration,” Cur. Bio, vol.13, no.19 pp.756-759, 2003.

G. Topman, O. Sharabani-Yosef, dan A. Gefen, “A standardized objective method for continuously measuring the kinematics of cultures covering a mechanically damaged site,” Medical Engineering and Physics, vol. 34, no.2, pp.225–232, 2012.

S. G. Park, J. H. Kim, Y. Xia, dan J. H. Sung, “Generation of reactive oxygen species in adiposederived stem cells: friend or foe?,” Expert Opin Ther Targets, vol.15, no.11, pp.1297–1306, 2011.

N. Liao et al., “Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion,” Stem Cell Res Ther, vol.10, no.306, 2019.

L. Y. Sun et al., “Antioxidants cause rapid expansion of human adipose-derived mesenchymal stem cells via CDK and CDK inhibitor regulation,” J Biomed Sci, vol.20, no.1, pp.53, 2013.

E. H. Yao, Y. Yu, dan N. Fukuda, “Oxidative stress on progenitor and stem cells in cardiovascular diseases,” Curr Pharm Biotechnol, vol.7, no.2, pp.101–108, 2006.

J. Case, D. A. Ingram, dan L. S. Haneline, “Oxidative stress impairs endothelial progenitor cell function,” Antioxid Redox Signal, vol.10, no.11, pp.1895–1907, 2008.

J. H.Kim, S. H. Park, S. G. Park, J. S. Choi, Y. Xia, dan J. H. Sung, “The pivotal role of reactive oxygen species generation in the hypoxia induced stimulation of adipose-derived stem cells,” Stem Cells Dev, vol.20, no.10, pp.1753–1761, 2011.




DOI: http://dx.doi.org/10.36722/sst.v5i4.404

Refbacks

  • There are currently no refbacks.


LP2M (Lembaga Penelitian dan Pengembangan Masyarakat)

Universitas AL-AZHAR INDONESIA, Lt.2 Ruang 207

Kompleks Masjid Agung Al Azhar

Jl. Sisingamangaraja, Kebayoran Baru

Jakarta Selatan 12110

Visitor