Pengaruh Tepung Kulit Pisang Kepok (Musa Paradisiaca L.) terhadap Aktivitas Antioksidan dan Sitotoksisitas pada Sel Kanker Payudara T-47D
Abstract
Abstrak – Kanker payudara masih menjadi permasalahan kesehatan yang kompleks. Salah satu upaya untuk mengatasi kanker adalah dengan konsumsi makanan tinggi kandungan antioksidan. Bahan pangan alternatif potensial adalah kulit pisang kepok yang mengandung senyawa fitokimia kaya antioksidan.Tepung kulit pisang belum banyak dimanfaatkan dan belum diteliti kandungan antioksidan serta potensi antikankernya. Penelitian ini bertujuan untuk melihat nilai aktivitas antioksidan, toksisitas, dan efek sitotoksisitas terhadap sel T-47D secara in vitro. Kulit dari pisang kepok matang direndam dalam larutan 1% Na2S2O5 selama 15 menit, dikeringkan dalam oven kabinet pada suhu 60ºC selama 7 jam, dihancurkan menggunakan blender, dan diayak menggunakan ayakan 80 mesh. Uji aktivitas antioksidan dilakukan dengan metode DPPH (2,2-difenil-1-pikrillhidrazil), uji toksisitas menggunakan metode BSLT (Brine Shrimp Lethality Test) serta uji sitotoksisitas dilakukan menggunakan MTT assay(3-(4, 5-dimetiltiazol-2-il)-2, 5-difeniltetrazolium bromid).Hasil aktivitas antioksidan didapatkan nilai IC50237,684 ppm yang menunjukkan aktivitas antioksidan bersifat sangat lemah, hasil uji toksisitas adalah LC50 4.665, 77 ppm yang menunjukkan tidak bersifat toksik bagi larva Artemia salina L. serta hasil IC50uji sitotoksisitas adalah 720.396 µg/ml yang menunjukkan efek sitotoksisitas lemah sehingga tepung kulit pisang kepok tidak berpotensi sebagai antikanker terhadap sel T-47D.
Abstract - Breast cancer still becomes a complex health problem. One of the attempts to overcome cancer is by consuming high antioxidants foods. Potential alternative food is kepok banana peels which contain phytochemical compounds rich in antioxidants. Banana peel flour has not been widely used and has not yet been studied its antioxidant content and anti-cancer potential. This study aimed to see the value of the antioxidant activity, toxicity, and cytotoxicity effects on T-47D cells in vitro. The peel of ripe kepok banana was soaked in 1% Na2S2O5 solution for 15 minutes, dried in a cabinet oven at 60ºC for 7 hours, grounded using a blender, and sieved using an 80 mesh sieve. The antioxidant activity test was carried out by the DPPH (2,2-diphenyl-1-pikrillhidrazil) method, the toxicity test used the BSLT (Brine Shrimp LethalityTest) method and the cytotoxicity test was carried out using the MTT assay (3- (4, 5-dimethyltiazol-2-il ) -2,5-diphenyl tetrazolium bromide). The antioxidant activity test results were IC50at 237,684 ppm which showed weak antioxidant activity, the toxicity test results were LC50at4,665.77ppm which showed no toxic effects for Artemia salina L. larvae and cytotoxicity test results were IC50at 720,396 µg / ml which showed a weak cytotoxicity effect indicating that kepok banana peel flour has no potential as an anticancer against T-47D cells.
Keywords –Tepung kulit pisang kepok, Antioksidan, Sitotoksisitas, BSLT, Kanker payudara, T-47D
Full Text:
PDFReferences
Kementrian Pertanian , Statistik Produksi Hortikultura 2014. Jakarta: Kementrian Pertanian Republik Indonesia, 2015.
N. Rajesh, "Medicinal Benefits of Musa Paradisiaca (Banana)," International Journal of Biology, vol. 2, no. 2, April 2017.
E. S. Lolodatu, "Kualitas Non Flanky Crackers Coklat Dengan Variasi Subtitusi Tepung Pisang Kepok Kuning (Musa paradisiaca forma typica)," Universitas Atma Jaya, Yogyakarta, Tesis 2015.
A. Sukowati and Sukarman, "Produksi Bioetanol Dari Kulit Pisang Melalui Hidrolisis Asam Sulfat," Universitas Lampung, Lampung, Tesis 2014.
M. E Rustanti, "Potensi Kulit Pisang Kepok Kuning (Musa paradisiaca L.) sebagai Bahan Tambahan Dalam Pembuatan Es Krim," Universitas Sanata Dharma, Yogyakarta, Tesis 2018.
Azza and Ferrial, "Nutritional and Anti-Nutrtional Composition of Banana Peels as Influenced by Microwave Drying Method," International Journal of Nutrition and Food Engineering , vol. 11, no. 12, 2017.
A. C. C. Egbuonu, W. O. Nneji, and C. A Ukasoanya, "Comparative Evaluation of Some Nutrient Contents and Antifungal Properties of Ground Musa paradisiaca (Plantain) Peels and Leaves," British Journal of Applied Science & Technology, vol. 19, no. 1, 2017.
M. Roy, A. Mukherjee, S. Mukherjee, and J. Biswas, "Phytochemicals as an adjuvant in leukemia therapy," semanticscholar, pp. 1-10, 2017.
E. Agama, J. A. S. Barajas, R. Veles, and G. A. G. Aguilar, "Potential of plantain peels flour ( Musa paradisiaca L.) as a source of dietary fiber and antioxidant compound," Journal of Food, vol. 14, no. 1, 2015.
International Agency for Reasearch on Cancer. (2019, Mei) Global Cancer Observatory. [Online]. https://gco.iarc.fr/today/data/factsheets/populations/360-indonesia-fact-sheets.pdf
K. B. Arun; F. Persia; P. S. Aswanthy; J. Chandran; M. S. Sajeev et all, "Plantain peel - a potential source of antioxidant dietary fibre for developing functional cookies," Journal Food Sci Technol, vol. 52, no. 10, Oktober 2015.
R. Futeri and Pharmayeni, "Substituting Wheat Flour with Banana Skin Flour from Mixture Various Skin Types of Banana on Making Donuts," International Journal on Advanced Science, Engineering and Information Technology, vol. 4, no. 2, 2014.
D. Tristantini, A. Ismawati, B. T. Pradana, and J. G. Jonathan, "Pengujian Aktivitas Antioksidan Menggunakan Metode DPPH pada Daun Tanjung (Mimusops elengi L)," in Prosiding Seminar Nasional Teknik Kimia "Kejuangan", Yogyakarta, 2016.
B. Arwan, "Uji Toksisitas Fraksi Ekstrak Etanol 70% Akar Parangromang (Boehmeria Virgata (Forst) Guill) Terhadap Larva Udang (Artemia Salina Leach) Dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT)," UIN Alauddin, Makassar, Skripsi 2017.
Food and Agriculture Organization of United Nations dan WHO. (2018) Codex Alimentari International Food standards. [Online]. http://www.fao.org/gsfaonline/docs/CXS_192e.pdf
M. Marshall and A. S. P. Grotheer. (2016) IFAS Extension. [Online]. https://edis.ifas.ufl.edu/pdffiles/FY/FY73100.pdf
D. H. Wardhani, A. e. Yuliana, and A. S. Dewi, "Natrium Metabisulfit Sebagai Anti-Browning Agent Pada Pencoklatan Enzimatik Rebung ORI (Bambusa Arundinacea)," Jurnal Aplikasi Teknologi Pangan, vol. 5, no. 4, 2016.
A. N. Syahruddin, I. A. Ibrahim, and Nurdiyanah S, "Identifikasi Zat Gizi dan Kualitas Tepung Kulit Pisang Raja (Musa Sapientum) dengan Metode Pengeringan Sinar Matahari dan Oven," Media Gizi Pangan, vol. 19, no. 1, 2015.
S. I. Behiry et all, "Antifungal and Antibacterial Activities of Musa paradisiaca L. Peel Extract: HPLC Analysis of Phenolic and Flavonoid Contents," MDPI, vol. 7, no. 4, Maret 2019.
Z. B. Ahmed et al., "Seasonal, gender and regional variations in total phenolic, flavonoid, and condensed tannins contents and in antioxidant properties from Pistacia atlantica ssp. leaves," Journal Pharmaceutical Biology, vol. 55, no. 1, 2017.
F. Shahidi and J. D.Yeo, "Insoluble-Bound Phenolics in Food," Molecules, vol. 21, no. 9, 2016.
A.E. Quirós-Sauceda; J.F. Ayala-Zavala; S.G. Sáyago-Ayerdi; R. Vélez-de la Rocha, "Added dietary fiber affects antioxidant capacity and phenolic compounds content," Journal of Applied Botany and Food Quality, vol. 87, 2014.
R. Chrisnasari and Sudono, "The Proximate and Phytochemical Properties of Red Pitaya (Hylocereus polyrhizus) Stem Flour and Its Potential Application as Food Products," Journal Tropical and Agricultural Science, vol. 42, no. 3, 2019.
S. Velumani, "Phytochemical Screening and Antioxidant Activity Of Banana Peel," IJARIIE, vol. 2, no. 1, 2016.
V. Kuete and T. Efferth, "African Flora Has the Potential to Fight Multidrug Resistance of Cancer," BioMed Research International, 2015.
M. Carocho and I.C.F.R Ferreira, "A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspective," Food and Chemical Toxicology, vol. 51, 2013.
D. A. Kim; H. S. Choi et all, "Tannic acid attenuates the formation of cancer stem cells by inhibiting NF-κB-mediated phenotype transition of breast cancer cells," American journal of cancer research, vol. 9, no. 8, 2019.
F. Wardati, K. Holil, and U. Syarifah, "Cytotoxicity Test on Breast Cancer Cell Lines T-47D treated with Pisang Kepok Peel Extract (Musa balbisiana)," Bioinformatics and Biomedical Research Journal, vol. 1, no. 3, 2018.
EBrglez Mojzer, Masa Knez Hrncic, Mojca Skerget, Zeljko Knez, and Urban Bren, "Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects," Molecules, vol. 21, no. 7, 2016.
Hugo Palafox-Carlos, Jesús Fernando Ayala-Zavala, and and Gustavo A González-Aguilar, "The Role of Dietary Fiber in the Bioaccessibility and Bioavailability of Fruit and Vegetable Antioxidants," Journal OF Food Science, vol. 76, no. 1, 2012.
Adedolapo Ibironke Falodun, Helen Ayo Omogie, and Olugbenga Olufemi Awolu, "[31] Falodun, A.I., Ayo-Omogie, H.N. and Awolu, O.O., 2019. Effect of Different Drying Techniques on the Resistant Starch, Bioactive Components, Physicochemical and Pasting Properties of Cardaba Banana Flour. Acta Universitatis Cibiniensis. Series E: Food," Acta Universitas Cinbiniesis Series E; FOOD TECHNOLOGY, vol. 23, no. 1, 2019.
Akmal Novrian Syahruddin, Irviani A. Ibrahi, and Nurdiyanah S, "Identifikasi Zat Gizi Dan Kualitas Tepung Kulit Pisang Raja (Musa sapientum) Dengan Metode Pengerigan Sinar Matahari Dan Oven ," Media Gizi Pangan, vol. 19, no. 1, 2015.
Xuehong Li, "Resistant Starch and Its Applications," Functional Starch and Applications in Food, 2018.
Devinder Dhingra, Mona Michael, Hradesh Rajput, and and R. T. Patil, "Dietary fibre in foods: a review," J Food Sci Technol, vol. 49, no. 3, 2011.
Perez-Hernandez LM et al., "Starch Digestion Enhances Bioaccessibility of Anti-Inflammatory Polyphenols from Borlotti Beans (Phaseolus vulgaris).," Nutrients. , vol. 12, no. 2, 2020.
Mulia Syafrida Sri Darmanti Muniffatul Izzati, "Pengaruh Suhu Pengeringan Terhadap Kadar Air, Kadar Flavonoid dan Aktivitas Antioksidan Daun dan Umbi Rumput Teki (Cyperus rotundus L.)," Bioma, vol. 20, no. 1, 2018.
van Tonder A, Joubert AM, and Cromarty AD, "Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays.," BMC Res Notes, vol. 8, no. 47, 2015.
S. H. Shahruzaman; M. F. Mustafa; S. Ramli; S. Maniam; S. Fakurazi & S. Maniam, "The cytotoxic effect and glucose uptake modulation of Baeckea frutescens on breast cancer cells," BMC Complementary and Alternative Medicine , vol. 19, 2019.
Niepel M et al., "Common and cell-type specific responses to anti-cancer drugs revealed by high throughput transcript profiling.," Nat Commun, vol. 8, no. 1, 2017.
Wahyu Widowati, Rahma micho Widyanto, Dian Laksmitawati, and Erawijantari pande putu, "Phytochemical, Free Radical Scavenging and Cytotoxic Assay of Cucumis Melo L. Extract and β-Carotene," Journal of Advanced Agricultural Technologies, vol. 2, no. 2, 2015.
DOI: http://dx.doi.org/10.36722/sst.v5i3.380
Refbacks
- There are currently no refbacks.
LP2M (Lembaga Penelitian dan Pengembangan Masyarakat)
Universitas AL-AZHAR INDONESIA, Lt.2 Ruang 207
Kompleks Masjid Agung Al Azhar
Jl. Sisingamangaraja, Kebayoran Baru
Jakarta Selatan 12110
This work is licensed under CC BY 4.0
