Investigating the Impact of Nitrogen Addition in Growing Media using Liquid Organic Fertilizer on Pigment and Protein Content of Spirulina sp.

Nadhira Saffana, Risa Swandari Wijihastuti, Nita Noriko

Abstract


Spirulina sp. is a highly promising microalgae with substantial protein and pigment content, holds great economic potential across various industries. However, the cultivation of Spirulina sp. often faces the challenge of high production costs, particularly in media formulation. To address this issue, the development of more affordable alternative media formulations is crucial. One potential approach involves utilizing a combination of liquid organic fertilizer derived from Canna indica waste and urea for cultivation. This study aims to determine the optimal ratio of the fertilizers mixture that influences protein and pigment synthesis. The study design employed a completely randomized design with seven treatment groups along with the control. The result shows that the treatment 2A (20 mL/L organic fertilizer; 125 ppm urea) demonstrated the highest dry biomass weight. Treatment 2C (22 mL/L organic fertilizer; 150 ppm urea) exhibited the best specific growth rate and carotenoid production, with the carotenoid production significantly differing from the control. Treatment 1A (20 mL/L organic fertilizer; 100 ppm urea) yielded the highest levels of chlorophyll and phycobiliproteins. Treatment 1B (20 mL/L organic fertilizer; 125 ppm urea) resulted in the highest protein production.

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W. Astuti, S. P. Astuti, Suripto and L. Japa, "Komunitas Mikroalga di Perairan Sungai dan Muara Sungai Pelangan Kecamatan Sekotong Kabupaten Lombok Barat," Jurnal Biologi Tropis, vol. 17, no. 1, pp. 76-86, 2017.

A. L. Lupatini, L. M. Colla, C. Canan and E. Colla, "Potential Application of Microalgae Spirulina platensis as a Protein Resource," Journal of the Science of Food and Agriculture, vol. 97, no. 3, pp. 724-732, 2016.

C. Osório, S. Machado, J. Peixoto, S. Bessada, F. B. Pimentel, R. C. Alves, M. Beatriz dan P. P. Oliveira, “Pigments Content (Chlorophylls, Fucoxanthin and Phycobiliproteins) of Different Commercial Dried Algae,",” Separations, pp. 1-14, 2020.

Dianursanti, Z. Taurina and C. M. Indraputri, "Optimization Growth of Spirulina platensis in Bean Sprouts Extract Medium with Urea Fertilizer for Phycocyanin Production as Antioxidant," in Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2017, 2018.

R. S. Wijihastuti, A. Lutfiah and N. Noriko, "Pengaruh Pertumbuhan Spirulina sp. terhadap Penggunaan Pupuk Organik Cair sebagai Media Tumbuh," Jurnal AL-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI, vol. 5, no. 4, pp. 202-207, 2020.

A. H. Pardosi, Irianto and Mukhsin, "Respons Tanaman Sawi terhadap Pupuk Organik Cair Limbah Sayuran pada Lahan Kering Ultisol," in Prosiding Seminar Nasional Lahan Suboptimal 2014, Palembang, 2014.

S. Aiba and T. Ogawa, "Assessment of Growth Yield of a Blue-Green Alga, Spirulina platensis, in Axenic and Continuous Culture," J. Gen Microbiol, vol. 102, no. 1, pp. 179-182, 1977.

R. A. Andersen, J. A. Berges, P. J. Harrison and M. M. Watanabe, "Appendix A—Recipes for Freshwater and Seawater Media," in Algal Culturing Technique, R. A. Andersen, Ed., London, Elsevier, 2005, pp. 435-436.

U. G. Schlösser, "SAG-Sammlung von Algenkulturen at the University of Göttingen Catalogue of Strains 1994," Bot. Acta, vol. 107, pp. 111-186, 1994.

D. R. Amanatin and T. Nurhidayati, "Pengaruh Kombinasi Konsentrasi Media Ekstrak Tauge (MET) dengan Pupuk Urea terhadap Kadar Protein Spirulina sp," Jurnal Sains & Seni ITS, vol. 2, no. 2, pp. 182-185, 2013.

N. R. Moheimani, M. A. Borowitzka, A. Isdepsky and S. F. Sing, "Standard Methods for Measuring Growth of Algae and Their Composition," in Algae for Biofuels and Energy,, M. A. Borowitzka and N. R. Moheimani, Eds., Dordrecht, Heidelberg, New York, London, Springer, 2013, pp. 269-270.

R. J. Ritchie, "Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents.," Photosynthesis Research, vol. 89, no. 1, pp. 27-41, 2006.

J. Strickland and T. Parsons, Practical Handbook of Seawater Analysis, Second Edition ed., Ottawa: Fisheries Research Board of Canada, 1972.

R. MacColl and D. Guard-Friar, Phycobiliproteins, Boca Raton: CRC Press, 1987.

O. Lowrey, N. Rosenbrough, A. Farr and R. RJ, "Protein Measurement with the Folin Phenol Reagent.," J. Biol Chem, vol. 1983, pp. 265-275, 1951.

T. E. Dorsey, P. McDonald and O. A. Roels, "Measurements of Phytoplankton-Protein Content with the Heated Biuret-Folin Assay," Journal of Phycology, vol. 14, no. 2, pp. 167-171, 1978.

A. Razaghi, A. Godhe and E. Albera, "Effects of nitrogen on growth and carbohydrate formation in Porphyridium cruentum," Open Life Sciences, vol. 9, no. 2, pp. 156-162, 2014.

M. Nagi, M. He, D. Li, T. Gebreluel, B. Cheng and C. Wang, "Utilization of tannery wastewater for biofuel production: New insights on microalgae growth and biomass production," Scientific reports, vol. 10, no. 1, pp. 1-14, 2020.

M. C. Matsudo, R. P. Bezerra, S. Sato, P. Perego, A. Converti and J. C. M. Carvalho, "Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source," Biochemical Engineering Journal, vol. 43, no. 1, pp. 52-57, 2009.

L. Gouveia, A. E. Marques, J. M. Sousa, P. Moura and N. M. Bandarra, "Microalgae – Source of Natural Bioactive Molecules as Functional Ingredients," Food Science and Technology Bulletin: Functional Foods, vol. 7, no. 2, pp. 21-37, 2010.

M. Rodrigues, L. Ferreira, A. Converti, S. Sato and J. Carvalho, "Fed-batch cultivation of Arthrospira (Spirulina) platensis: Potassium nitrate and ammonium chloride as simultaneous nitrogen sources," Bioresource Technology, vol. 101, p. 4491–4498, 2010.

S. Sedjati, G. Santosa, E. Yudiati, E. Supriyantini, A. Ridlo and F. Kimberly, "Chlorophyll and Carotenoid Content of Dunaliella salina at Various Salinity Stress and Harvesting Time," in 4th International Conference on Tropical and Coastal Region Eco Development, 2019.

F. K. El-Baz, A. Aboul-Enein, G. E. Baroty, A. M. Youssef and H. H. A. E. Baky, "Anticarcinogenic Activity of Algal Extracts," Journal of Medical Sciences, vol. 2, pp. 243-251, 2002.

H. H. A. El-Baky, G. S. El-Baroty and E. M. Mostafa, "Optimazation Growth of Spirulina (Arthrospira) platensis in Photobioreactor Under Varied Nitrogen Concentration for Maximized Biomass, Carotenoids and Lipis Contents," Recent Patents on Food, Nutritions & Agriculture, vol. 10, 2019.

P. Vaughan and J. Donohue, "The Structure of Urea. Interatomic Distances and Resonance in Urea and Related Compounds," Acra Cryst, vol. 5, pp. 530-535, 1952.

M. Cuaresma, C. Casal, E. Forján and C. Vílchez, "Productivity and selective accumulation of carotenoids of the novel extremophile microalga Chlamydomonas acidophila grown with different carbon sources in batch systems," Journal of Industrial Microbiology and Biotechnology, vol. 1, no. 1, pp. 167-177, 2011.

F. R. Nurani, E. D. Masithah and A. S. Mubarak, "Pengaruh Konsentrasi Pupuk Azolla pinata Terhadap Pertumbuhan Populasi Spirulina platensis," Jurnal Ilmiah Perikanan dan Kelautan, vol. 4, no. 1, pp. 39-44, 2012.

V. K. Kannaujiya, D. Kumar, V. Singh and R. P. Sinha, "Advances in phycobiliproteins research: innovations and commercialization," in Natural Bioactive Compounds: Technological Advancements, Academic Press, 2021, pp. 57-81.

M. I. Khazi, Z. Demirel and M. C. Dalay, "Evaluation of growth and phycobiliprotein composition of cyanobacteria isolates cultivated in different nitrogen sources," Journal of Applied Phycology, vol. 30, pp. 1513-1523, 2018.

J. P. Pandey and A. Tiwari, "Optimization of Biomass Production of Spirulina maxima," Journal of Algal Biomass Utilization, vol. 1, no. 2, pp. 20-32, 2010.

R. A. Soni, K. Sudhakar and R. S. Rana, "Comparative Study on the Growth Performance of Spirulina platensis on Modifying Culture Media," Energy Reports, vol. 5, pp. 327-336, 2019.

S. F. Mohsenpour and N. Willoughby, "Effect of CO2 aeration on cultivation of microalgae in luminescent photobioreactors," Biomass and Bioenergy, vol. 85, pp. 168-177, 2016.

Y. Li, M. Horsman, B. Wang, N. Wu and C. Q. Lan, "Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans," Applied Microbiology and Biotechnology, vol. 81, no. 4, pp. 629-636, 2008.




DOI: http://dx.doi.org/10.36722/exc.v1i1.2348

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