Detection of Secondary Metabolites in Cucumber (Cucumis sativus) Leaves and Its Potential as Candidates for Acne Drug Using Histochemical Analysis and In Silico Study

Devi Indrayani Fitri Asari, Ramizard Rafsanjani, Muchammad Faruq Vikriansyah, Abdullah Rozzen Muzayyan, Nurul Jadid Mubarakati, Gatra Ervi Jayanti

Abstract


Acne is a facial skin problem that is generally experienced by 75-80% of adolescents as a result of excessive production of oil glands and sweat, inflammatory mediators. The acnecausing bacteria, Propionibacterium acnes, involves KAS III and JNK 1 proteins, so it is expected to be an effective anti-inflammatory and antibacterial strategy. Cucumber leaves are known to have biological activity as a candidate for acne medication. This study aims to detect secondary metabolites in cucumber leaves through histochemical and in silico analysis. Histochemical analysis was carried out by preparing fresh leaves through lower leaf incisions by dropping secondary metabolite detection reagents and then observing the color changes microscopically. The in silico test aims to determine the physicochemical properties, pharmacokinetic properties and interactions of the active compounds with KAS III and JNK1 as acne drug targets through molecular docking. Histochemical analysis showed that cucumber leaves positively contained secondary metabolites, namely terpenoids, flavonoids, alkaloids, tannins, and phenols. Meanwhile, it was known that isovitexin, cucurbitacin B & C had fairly good physicochemical properties, but only isovitexin and cucurbitacin C had the best pharmacokinetic properties. Based on the results of molecular docking, there are the same amino acid bonds between isovitexin and the control at the JNK1 receptor, namely the amino acids Ile:32, Val:40 and Leu:168 and the binding affinity value is low so it is predicted to be the most effective in inhibiting JNK1. Therefore, cucumber leaves open up a new potential as a candidate for natural acne preparations.

Keywords: acne, cucumber, secondary metabolites, histochemical, in silico


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References


Katzman M. and AC Logan. 2007. Acne vulgaris: Nutritional factors may be influencing psychological sequelae. Med Hypotheses, 69(5):1080-4

Strauss J.S., D.P. Krowchuk, J.J. Leyden, A.W. Lucky, A.R. Shalita, and A.C. Siegfried. 2007. Guidelines of Care For Acne Vulgaris Management. Journal of American Academy of Dermatology, 56:651-63

Dasom C., J. Kim, D. Jeon, HC. Shin, dan Y. Kim. 2019. Target Proteins of Phloretin for Its AntiInflammatory and Antibacterial Activities Against Propionibacterium acnes Induced Skin Infection. Journal of Molecules; 24(7): 1319E

Targhetti, E.A. 2013. The role of inflammation in pathology of acne. J Clin Aesthet Dermatol, 6(9):27-35

Viogenta, P., Samsuar, dan A.F.Y. Utama. 2017. “Fraksi Kloroform Ekstrak Buah Mentimun (Cucumis sativus L.) Sebagai Anti Bakteri terhadap Staphylococcus epidermidis”. Jurnal Kesehatan 8(2): 165-169

Tuama, A.A. dan A.A. Mohammed. 2019. Phytochemical screening and in vitro antibacterial and anticancer activities of the aqueous extract of Cucumis sativus. Saudi J Biol Sci, 26: 600-604, https://doi.org/10.1016/j.sjbs.2018.07.012.

Rajasree, R.S. P.I. Sibi, F. Francis, dan H. 2016. William. Phytochemicals of Cucurbitaceae family. Int J Pharmacogn Phytochem Res, 8:113-123

Balqis, U., Frengky, N. Azzahrawani, Hamdani, D. Aliza, dan, T. Armansyah. 2016. “Efikasi Mentimun (Cucumis sativus L.) Terhadap Percepatan Penyembuhan Luka Bakar (Vulnus ombustion) Derajat IIIB Pada Tikus Putih (Rattus norvegicus)”. Jurnal Medika Veterinaria 10 (2) : 90-93

Novelina, S. A.S. Satyaningtijas, S. Agungpriyono, H. Setijanto, dan K. Sigit. 2010. “Morfologi dan Histokimia Kelenjar Mandibularis Walet linchi (Collocalia linchi) Selama Satu Musim Berbiak dan Bersarang”. Jurnal Kedokteran Hewan, 4(1):194-202

Krovat, T.S and T. Langer. 2005. Recent Advances in Docking and Scoring. Curr. Comput. Aided Drug Des, 1(1): 93-102

Nogrady T. dan D. F. Weaver. 2005. Medicinal Chemistry: A Molecular and Biochemical Approach. Oxford University Press: New York

Kalita, J., D. Chetia, and M. Rudrapal, 2019. Molecular docking, drug-likeness studies and ADMET prediction of quinoline imines for antimalarial activity. J. Med. Chem. Drug Des., 2(1): 1-7

Velázquez, J. L., F. Durán-Verdugo, A. Valdés-Jiménez, G. Núñez-Vivanco, and J. Caballero. 2020. LigRMSD: A web server for automatic structure matching and RMSD calculations among identical and similar compounds in protein-ligand docking,’ Bioinformatics, 36(9):2912–2914.

Andriya N., 2016. Analisis Struktur Anatomi dn Histokimia Tiga Varietas Kumis kucing (Orthosiphon aristatus (Blume) Miq). SKRIPSI. Departemen Biologi, Institut Pertanian Bogor. Bogor.

Rupa D. 2015. Identifikasi Struktur Sekretori dan Analisis Histokimia dan Uji Fitokimia Tumbuhan Obat Anti-infeksi di Kawasan Taman Nasional Bukit Dua belas Jambi. TESIS. Institut Pertanian. Bogor

Prasojo S.L., F.A. Hartanto N. Yuniarti Z. Ikawati dan E.P. Istyastono. 2010. Docking of 1-Phenylsulfonamide-3Trifluoromethyl-5-parabromophenyl-phyrazol to cyclooxyganase-2 using PLANTS. Indonesian Journal of Chemistry. 10(3):348-51

Trianto, Dini D. dan I. Serafinah. 2018. Morfologi, Anatomi dan Uji Histokimia Rimpang Curcuma aeruginosa Roxb, Curcuma Longa L. dan Curcuma heyneana Valeton dan Zijp. Jurnal Ilmu-Ilmu Hayati

Anisa K. T. Rahayu. dan A. Hayati. 2018. Profil metabolit sekunder daun Tin (Ficus carica) melalui analisis histokimia dan deteksi flavonoid dengan metode kromatografi lapis tipis (KLT). E-Jurnal ilmiah SAINS ALAMI Vol.1 No.1 Hal 104-110

Dorly, B.A. Wiryo, I. Nurfaizah & RR.S. Nidyasari. 2015. Struktur Sekretori dan Uji Histokimia Tumbuhan Obat Anggota Suku Astraceae di Hutan Pendidikan Gunung Walat. Seminar Nasional XII Pendidikan Biologi FKIP UNS.

Sofiyanti, N., D. Iriani & A.R. Lestari. 2022. Kajian Anatomi-Histokimia Tangkai Daun dan Karakteristik Epidermis Pucuk Merah (Syzygium myrtifolium Walp.-Myrtaceae). Buletin Anatomi dan Fisiologi, 7(2):83-90

Cheon, D., J. Kim, D. Jeon, HC.Shin and Y. Kim. Target Proteins of Phloretin for Its AntiInflammatory and Antibacterial Activities Against Propionibacterium acnes-Induced Skin Infection. Molecules. 3;24(7):1319. doi: 10.3390/molecules24071319

Susanti, N. M. P., N. P. L. Laksmiani, N. K. M. Noviyanti, K. M. Arianti, dan I K. Duantara. 019. Molecular Docking Terpinen-4-Ol Sebagai Antiinflamasi Pada Aterosklerosis Secara In Silico. Jurnal Kimia (Journal of Chemistry), [S.l.], 13 (2): 221-228. ISSN 2599-274




DOI: http://dx.doi.org/10.36722/exc.v1i1.2232

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